GENOME-WIDE IDENTIFICATION OF RAPIDLY EVOLVING GENES IN XYLELLA FASTIDIOSA: KEY ELEMENTS IN THE SYSTEMATIC IDENTIFICATION OF HOST STRAINS, AND IN THE SEARCH FOR PLANT-HOST PATHOGENICITY CANDIDATE GENES Project Leader:
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چکیده
INTRODUCTION Genomic research provides an extraordinarily powerful new tool for solving applied problems. It can be extremely effective at resolving evolutionary relationships and can be used to extend earlier work defining the interrelationships of the plant-host strains of Xylella fastidiosa (see Hendson et al. 2001). Similarly, it can provide effective methods for identifying these host strains. Unambiguous identification is of considerable importance for understanding the epidemiology of Pierce’s disease and the other plant diseases caused by this bacterium. This has been approached using a variety of DNA based methods (Banks et al. 1999; Hendson et al. 2001; Rodrigues et al. 2003; Meinhardt et al. 2003;); however, an effective methodology for identifying the plant-host strains, including when they are mixed together, has yet to be developed.
منابع مشابه
A GENOME-WIDE APPROACH TO PLANT-HOST PATHOGENICITY IN XYLELLA FASTIDIOSA: MULTIGENIC METHODS FOR IDENTIFYING STRAINS, FOR STUDYING THE ROLE OF INTER-STRAIN RECOMBINATION, AND FOR IDENTIFYING PATHOGENICITY CANDIDATE GENES Project Leader:
We have developed a multilocus sequence typing (MLST) system for identifying the known pathovars of Xylella fastidiosa (including subsp. fastidiosa that causes Pierce’s disease). This identification system is based on allelic variation at seven housekeeping genes (holC, nuoL, leuA, gltT, cysG, petC and lacF) and a public MLST database has been established at www.mlst.net (see Scally et al. 2005...
متن کاملBIOLOGICAL CONTROL OF PIERCE’S DISEASE WITH NON-PATHOGENIC STRAINS OF XYLELLA FASTIDIOSA Project Leader:
This project is to construct and test nonpathogenic strains of X. fastidiosa derived from a pathogenic Pierce’s disease strain for competitive exclusion of the pathogen in grapevines. Potential virulence genes were selected from comparative genome sequence analyses as well as DNA macroarray studies of differential gene expression. A more comprehensive analysis of differential gene expression wi...
متن کاملDNA MICROARRAY AND MUTATIONAL ANALYSIS TO IDENTIFY VIRULENCE GENES IN XYLELLA FASTIDIOSA Project Leader:
The identification of genetic factors that enable Xylella fastidiosa to express Pierce’s disease symptoms is essential to the further development of several disease control strategies. One such control strategy is to use non-pathogenic derivatives of the Pierce’s disease pathogen itself to competitively exclude pathogenic strains in grapevines. We have made progress in the development of geneti...
متن کاملANALYSIS OF XYLELLA FASTIDIOSA TRANSPOSON MUTANTS AND DEVELOPMENT OF AN EN PLANTA XYLELLA FASTIDIOSA PLASMID VECTOR Project Leader: Cooperator:
We have developed an autonomously replicating Xylella fastidiosa (Xf)/E. coli plasmid that efficiently transforms Xf; unfortunately this plasmid was not stably maintained in Xf cells en planta or without antibiotic selection in vitro. Another plasmid, containing an Xf native plasmid, a Kan cassette and cloned in pUC18 was also constructed and shown to be unstable without antibiotic selection. 1...
متن کاملROLE OF UNIQUE GENES OF XYLELLA FASTIDIOSA GRAPE STRAIN IN HOST SPECIFICITY AND VIRULENCE TO GRAPE AND TO INSECT USING MICROARRAY Project Leader:
Xylella fastidiosa (Xf) is a group of genetically similar strains that infect a wide range of plants. We hypothesized that differing genetic factors among the strains determine the ability of a strain to infect a particular host plant. To better understand what makes grape a good host for all grape strains but not for strains such as oleander and almond that cannot colonize grape, we conducted ...
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تاریخ انتشار 2007